ICAM-1 (phospho Tyr512) Cell Based ELISA Kit (A102710)

ICAM-1 (phospho Tyr512) Cell Based ELISA Kit

Cell-based (qualitative)

This ICAM-1 (phospho Tyr512) Cell Based ELISA Kit allows for the detection of ICAM-1 (phospho Tyr512) and the effects that certain stimulation conditions have on ICAM-1 (phospho Tyr512) expression in different cell lines. Qualitative determination of ICAM-1 (phospho Tyr512) concentration is achieved by an indirect ELISA format. In essence, the ICAM-1 (phospho Tyr512) is captured by Anti-ICAM-1 (phospho Tyr512) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this ICAM-1 (phospho Tyr512) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-ICAM-1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 6 months.

Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2