Anti-IDH2 Antibody [ARC0567] (A308463)

Name: Anti-IDH2 Antibody [ARC0567]
Brand: Antibodies.com
SKU: A308463

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This product is discontinued

Anti-IDH2 Antibody [ARC0567] (A308463) has been discontinued and is no longer available.

View all Anti-IDH2 Antibodies.

Specifications

Name

Anti-IDH2 Antibody [ARC0567]

Description

Rabbit monoclonal [ARC0567] antibody to IDH2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 353-452 of human IDH2 (P48735).

Sequence

RHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0567

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

D2HGA2, ICD-M, IDH, IDHM, IDHP_HUMAN, IDP, IDPM, Isocitrate dehydrogenase 2 (NADP+), mitochondrial, Isocitrate dehydrogenase [NADP], mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IDH2 Antibody [ARC0567] (A308463)

Western blot analysis of extracts of various cell lines, using Anti-IDH2 Antibody [ARC0567] (A308463) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-IDH2 Antibody [ARC0567] (A308463)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-IDH2 Antibody [ARC0567] (A308463) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-IDH2 Antibody [ARC0567] (A308463)

Immunofluorescence analysis of C6 cells using Anti-IDH2 Antibody [ARC0567] (A308463) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-IDH2 Antibody [ARC0567] (A308463)

Immunofluorescence analysis of NIH-3T3 cells using Anti-IDH2 Antibody [ARC0567] (A308463) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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