Anti-IFITM1 Antibody [ARC55317] (A307414)

Name: Anti-IFITM1 Antibody [ARC55317]
Brand: Antibodies.com
SKU: A307414

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This product is discontinued

Anti-IFITM1 Antibody [ARC55317] (A307414) has been discontinued and is no longer available.

View all Anti-IFITM1 Antibodies.

Specifications

Name

Anti-IFITM1 Antibody [ARC55317]

Description

Rabbit monoclonal [ARC55317] antibody to IFITM1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence within amino acids 1-80 of human CD225/IFITM1 (NP_003632.4).

Sequence

MHKEEHEVAVLGPPPSTILPRSTVINIHSETSVPDHVVWSLFNTLFLNWCCLGFIAFAYSVKSRDRKMVGDVTGAQAYAS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55317

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

9-27, CD 225, CD 225 antigen, CD225, CD225 antigen, Dispanin subfamily A member 2a, DSPA2a, IFI 17, IFI17, IFM1_HUMAN, Interferon induced protein 17, Interferon induced transmembrane protein 1, interferon induced transmembrane protein 1 (9-27), Interferon inducible protein 9-27, Interferon-induced protein 17, Interferon-induced transmembrane protein 1, Interferon-inducible protein 9-27, Leu 13, Leu 13 antigen, Leu-13 antigen, LEU13

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IFITM1 Antibody [ARC55317] (A307414)

Western blot analysis of various lysates, using Anti-IFITM1 Antibody [ARC55317] (A307414) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-IFITM1 Antibody [ARC55317] (A307414)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-IFITM1 Antibody [ARC55317] (A307414) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-IFITM1 Antibody [ARC55317] (A307414)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-IFITM1 Antibody [ARC55317] (A307414) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-IFITM1 Antibody [ARC55317] (A307414)

Immunofluorescence analysis of K-562 using Anti-IFITM1 Antibody [ARC55317] (A307414) at a dilution of 1:100(40x lens). DAPI was used to stain the cell nuclei (blue).

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