Mouse monoclonal [Mc24-2E11] antibody to Human IgA Heavy Chain.
Specificity
This antibody recognises human immunoglobulin alpha heavy chains. No cross reactivity observed with IgG, IgM or free kappa and lambda light chains.
Applications
WB, IP, IHC-Fr, ELISA
Reactivity
Human
Immunogen
Reduced and alkylated human IgA.
Host
Mouse
Clonality
Monoclonal
Clone ID
Mc24-2E11
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of ascites.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in TRIS Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human IgA heavy chain antibody, clone Mc24-2E11 recognizes human immunoglobulin alpha heavy chains. No cross reactivity observed with IgG, IgM or free kappa and lambda light chains.
Anti-Human IgA Heavy Chain Antibody [Mc24-2E11] (A281069)
Sandwich ELISA analysis of human IgA binding using Anti-Human IgA Heavy Chain Antibody [Mc24-2E11] (A281069) as a capture reagent and biotinylated Anti-Human IgA Antibody [15D6] (A279032) as a detection reagent with Native Human Secretory IgA Protein (A320429) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions.
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