Anti-Duck IgY Light Chain Antibody [14A3] (A279017)

Anti-Duck IgY Light Chain Antibody [14A3]

Mouse monoclonal [14A3] antibody to Duck IgY Light Chain.

This antibody recognises the light chain of duck immunoglobulin, expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479), reveals the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also reveals a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 using chBAFF-Flag (B cell activating factor).recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese. Mouse anti Duck IgY Light Chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

WB, ELISA, Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Duck, Goose

This antibody does not cross react with Chicken

Purified Pekin duck yolk IgY.

Mouse

Monoclonal

14A3

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes the light chain of duck immunoglobulin, expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479), reveals the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also reveals a high percentage of CD8+ /14A3+ cells in duck bursa (Kothlow et al. 2005). The recognition of B cells by 14A3 using chBAFF-Flag (B cell activating factor).Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese.Mouse anti Duck IgY Light Chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting.