This antibody recognizes Ikaros, a transcription factor expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.

Reactivity

Mouse, Human

Immunogen

Recombinant fragment corresponding to the C terminus of human Ikaros protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

4E9

Isotype

IgG1

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

CLL associated antigen KW 6, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1, Ikaros (zinc finger protein), IKAROS family zinc finger 1 (Ikaros), Ikaros family zinc finger protein 1, Ikzf1, IKZF1_HUMAN, LYF1, Lymphoid transcription factor LyF-1, PRO0758, Zinc finger protein subfamily 1A 1, Zinc finger protein subfamily 1A 1 (Ikaros), Zinc finger protein, subfamily 1A, member 1, ZNFN1A1

Isotype Controls