Anti-IKB alpha Antibody [ARC0194] (A307325)

Name: Anti-IKB alpha Antibody [ARC0194]
Brand: Antibodies.com
SKU: A307325

Enlarge Image

This product is discontinued

Anti-IKB alpha Antibody [ARC0194] (A307325) has been discontinued and is no longer available.

View all Anti-IKB alpha Antibodies.

Specifications

Name

Anti-IKB alpha Antibody [ARC0194]

Description

Rabbit monoclonal [ARC0194] antibody to IKB alpha.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human I?Ba (P25963).

Sequence

MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0194

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, IKBalpha, IKBA_HUMAN, MAD 3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-IKB alpha Antibody [ARC0194] (A307325)

Western blot analysis of extracts of various cell lines, using Anti-IKB alpha Antibody [ARC0194] (A307325) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-IKB alpha Antibody [ARC0194] (A307325)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-IKB alpha Antibody [ARC0194] (A307325) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-IKB alpha Antibody [ARC0194] (A307325)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-IKB alpha Antibody [ARC0194] (A307325) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-IKB alpha Antibody [ARC0194] (A307325)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-IKB alpha Antibody [ARC0194] (A307325) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.