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Anti-IKB alpha (phospho Ser32) Antibody (A306172)

Western Blot - Anti-IKB alpha (phospho Ser32) Antibody (A306172) - Antibodies.com
This product is discontinued

Anti-IKB alpha (phospho Ser32) Antibody (A306172) has been discontinued and is no longer available.

View all Anti-IKB alpha Antibodies.

Name
Anti-IKB alpha (phospho Ser32) Antibody
Description
Rabbit polyclonal antibody to IKB alpha (phospho Ser32).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S32 of human NFKBIA (NP_065390.1).
Sequence
HDSGL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, IKBalpha, IKBA_HUMAN, MAD 3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-IKB alpha (phospho Ser32) Antibody (A306172) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-IKB alpha (phospho Ser32) Antibody (A306172)
Western blot analysis of extracts of various cell lines, using Anti-IKB alpha (phospho Ser32) Antibody (A306172) at 1:1,000 dilution. Both HeLa cells and NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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