Anti-IL-1 alpha Antibody (A89441)

Name: Anti-IL-1 alpha Antibody
Brand: Antibodies.com
SKU: A89441
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to IL-1 alpha for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-IL-1 alpha Antibody

Description

Rabbit polyclonal antibody to IL-1 alpha.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 113-267 of human IL-1 alpha (NP_000566.3).

Sequence

SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQIL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

31 kDa / 21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BAF, FAF, Hematopoietin 1, Hematopoietin-1, IL 1 alpha, Il 1a , Il-1a, IL1 , IL1 alpha, IL1A, IL1A_HUMAN, IL1F1 , Ilia , Interleukin 1 alpha, Interleukin 1 alpha precursor , Interleukin-1 alpha, Interleukin1 alpha , LAF, LEM, OTTHUMP00000203692, OTTMUSP00000016430, Preinterleukin 1 , Preinterleukin 1 alpha, Pro interleukin 1, Pro interleukin 1 alpha, Prointerleukin 1, RP23-160G19.8

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IL-1 alpha Antibody (A89441)

Western blot analysis of extracts of Rat lung, using Anti-IL-1 alpha Antibody (A89441) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-IL-1 alpha Antibody (A89441)

Western blot analysis of extracts of Recombinant Human IL-1 alpha/IL-1 alpha/IL1F1 Protein, using Anti-IL-1 alpha Antibody (A89441) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-IL-1 alpha Antibody (A89441)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-IL-1 alpha Antibody (A89441) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-IL-1 alpha Antibody (A89441)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-IL-1 alpha Antibody (A89441) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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