Anti-IL-10 Antibody [ARC0650] (A307426)

Name: Anti-IL-10 Antibody [ARC0650]
Brand: Antibodies.com
SKU: A307426
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0650] antibody to IL-10 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-IL-10 Antibody [ARC0650]

Description

Rabbit monoclonal [ARC0650] antibody to IL-10.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL10 (P22301).

Sequence

MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAEN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0650

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CSIF, Cytokine synthesis inhibitory factor, GVHDS, IL 10, IL10, IL10A, IL10_HUMAN, Interleukin 10 , Interleukin-10, Interleukin10, MGC126450, MGC126451, T cell growth inhibitory factor, T-cell growth inhibitory factor, TGIF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IL-10 Antibody [ARC0650] (A307426)

Western blot analysis of extracts of various cell lines, using Anti-IL-10 Antibody [ARC0650] (A307426) at 1:1,000 dilution. THP-1 cells were treated by LPS and Brefeldin A for different times of stimulation. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-IL-10 Antibody [ARC0650] (A307426)

Western blot analysis of extracts of Rat thymus, using Anti-IL-10 Antibody [ARC0650] (A307426) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-IL-10 Antibody [ARC0650] (A307426)

Immunofluorescence analysis of Jurkat cells using Anti-IL-10 Antibody [ARC0650] (A307426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-IL-10 Antibody [ARC0650] (A307426)

Immunofluorescence analysis of rat thymus using Anti-IL-10 Antibody [ARC0650] (A307426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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