Anti-IL-2 Receptor alpha Antibody [K231.3B2] (A280791)

Anti-IL-2 Receptor alpha Antibody [K231.3B2]

Mouse monoclonal [K231.3B2] antibody to IL-2 Receptor alpha.

This antibody recognises porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Ra), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity ß monomer and the low affinity a monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens. Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations. CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Porcine

Con A activated porcine peripheral blood lymphocytes.

Mouse

Monoclonal

K231.3B2

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD25, clone K231.3B2 recognizes porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Ra), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity ß monomer and the low affinity a monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Lunney et al. 1994).Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations (Bailey et al. 1992). CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen (Bullido et al. 1999).