Specificity
Mouse anti Human IL-27α, clone H08-5C8, recognizes IL-27α, a subunit of IL-27. IL-27 is a 62 kDa member of the Il-6/IL-12 family of long type I cytokines. IL-27 is heterodimeric group 2 receptor composed of Epstein-Barr Virus induced gene 3 (EBI3) and IL-27α. EBI3 is a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23 and IL-27α is a 28 kDa glycoprotein that is related to the p35 of chain IL-12.IL-27 has broad range of pro- and anti-inflammatory properties, mediating its effects via its gp130-associated cytokine receptor and JAK/STAT signalling cascades. It can alter T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells and has diverse effects on innate immune cells. In vivo, it is reported to play an important role in immune regulation, as mice with defects in IL-27 or its receptor display enhanced immune responses (Batten & Ghilardi 2007).
