Anti-IL-33 Antibody [ARC58115] (A309924)

Name: Anti-IL-33 Antibody [ARC58115]
Brand: Antibodies.com
SKU: A309924
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC58115] antibody to IL-33 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-IL-33 Antibody [ARC58115]

Description

Rabbit monoclonal [ARC58115] antibody to IL-33.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000, ICC/IF: 1:100-1:500

Reactivity

Human

Immunogen

Recombinant protein of human IL33.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58115

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C9orf26, CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27, DVS27 related protein, IL 1F11, IL 33, IL-1F11, IL1F11, IL33, IL33_HUMAN, Interleukin 1 family member 11, Interleukin 33, INTERLEUKIN 33 NFHEV, Interleukin 33 precursor, Interleukin-1 family member 11, Interleukin-33, Interleukin-33 (109-270), Interleukin33, NF HEV, NF-HEV, NFEHEV, NFHEV, Nuclear factor for high endothelial venules, Nuclear factor from high endothelial venules, OTTHUMP00000021041, RP11 575C20.2, RP11-575C20.2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-IL-33 Antibody [ARC58115] (A309924)

Western blot analysis of 293F, using Anti-IL-33 Antibody [ARC58115] (A309924) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.

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Figure 2: Immunohistochemistry - Anti-IL-33 Antibody [ARC58115] (A309924)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-IL-33 Antibody [ARC58115] (A309924) at a dilution of 1:900 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-IL-33 Antibody [ARC58115] (A309924)

Immunofluorescence analysis of 293F-IL-33 and 293F cells using Anti-IL-33 Antibody [ARC58115] (A309924) at a dilution of 1:400 (40x lens). DAPI was used to stain the cell nuclei (blue).

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