Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016)

Name: Anti-IL-7 Antibody [ARC57481 + ARC57489]
Brand: Antibodies.com
SKU: A310016
Price: 470.0 USD
Availability: InStock

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$470

Rabbit Oligoclonal [ARC57481 + ARC57489] antibody to IL-7 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-IL-7 Antibody [ARC57481 + ARC57489]

Description

Rabbit Oligoclonal [ARC57481 + ARC57489] antibody to IL-7.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human IL7.

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC57481 + ARC57489

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 - 35 kDa / 17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

IL 7, IL7, IL7 nirs variant 6, IL7_HUMAN, Interleukin 7, Interleukin-7, Interleukin7, LP-1, Lymphopoietin 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016)

Western blot analysis of Recombinant Human IL-7 Protein, using Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016)

Western blot analysis of various lysates, using Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-IL-7 Antibody [ARC57481 + ARC57489] (A310016) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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