Anti-IMPDH2 Antibody [ARC1479] (A308284)

Name: Anti-IMPDH2 Antibody [ARC1479]
Brand: Antibodies.com
SKU: A308284
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1479] antibody to IMPDH2 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-IMPDH2 Antibody [ARC1479]

Description

Rabbit monoclonal [ARC1479] antibody to IMPDH2.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human IMPDH2 (P12268).

Sequence

QANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1479

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

56 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

IMDH2_HUMAN, IMP (inosine monophosphate) dehydrogenase 2, IMP dehydrogenase 2, IMP oxireductase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH-II, Inosine 5' monophosphate dehydrogenase 2, Inosine 5' phosphate dehydrogenase 2, Inosine monophosphate dehydrogenase type II, Inosine-5''-monophosphate dehydrogenase 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-IMPDH2 Antibody [ARC1479] (A308284)

Western blot analysis of various lysates, using Anti-IMPDH2 Antibody [ARC1479] (A308284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-IMPDH2 Antibody [ARC1479] (A308284)

Western blot analysis of extracts from wild type(WT) and IMPDH2 knockout (KO) NIH/3T3(KO) cells, using Anti-IMPDH2 Antibody [ARC1479] (A308284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunofluorescence - Anti-IMPDH2 Antibody [ARC1479] (A308284)

Immunofluorescence analysis of C6 cells using Anti-IMPDH2 Antibody [ARC1479] (A308284) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-IMPDH2 Antibody [ARC1479] (A308284)

Immunofluorescence analysis of NIH-3T3 cells using Anti-IMPDH2 Antibody [ARC1479] (A308284) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).