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Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946)

Western Blot - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) - Antibodies.com Western Blot - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) - Antibodies.com Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Indoleamine 2, 3-dioxygenase for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Indoleamine 2, 3-dioxygenase Antibody
Description
Rabbit polyclonal antibody to Indoleamine 2, 3-dioxygenase.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-403 of human IDO1 (NP_002155.1).
Sequence
MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
3-dioxygenase, I23O1_HUMAN, IDO, IDO 1, IDO-1, IDO1, INDO, indolamine 2,3 dioxygenase, Indole 2 3 dioxygenase, indoleamine 2 3 dioxygenase, indoleamine 2 3 dioxygenase 1, Indoleamine 2,3-dioxygenase 1, Indoleamine pyrrole 2 3 dioxygenase, Indoleamine-pyrrole 2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946)
Western blot analysis of extracts of SKOV3 cells, using Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946)
Western blot analysis of extracts of A-549 cells, using Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) at 1:500 dilution. A-549 cells were treated by hIFN-gamma(100 ng/ml) at 37°C for 48 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946)
Immunofluorescence analysis of U2OS cells using Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Indoleamine 2, 3-dioxygenase Antibody (A8946)? Please let us know so that we can list the citation on this page.

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