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Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567)

Western Blot - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.com Western Blot - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.com Immunohistochemistry - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.com Immunohistochemistry - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.com Immunofluorescence - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.com
This product is discontinued

Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) has been discontinued and is no longer available.

View all Anti-Integrin beta 1 Antibodies.

Name
Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468]
Description
Rabbit Oligoclonal [ARC52467 + ARC52468] antibody to Integrin beta 1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:2,000-1:20,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-728 of human Integrin-ß1/CD29 Rabbit PolymAb® (NP_002202.2).
Sequence
MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD
Host
Rabbit
Clonality
Oligoclonal
Clone ID
ARC52467 + ARC52468
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
beta1 integrin, CD29, Fibronectin receptor subunit beta, FNRB, Glycoprotein IIa, GP IIa, GPIIA, Integrin beta-1, Integrin subunit beta 1, integrin VLA-4 beta subunit, Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12), ITB1_HUMAN, ITGB1, MDF2, MSK12, OTTHUMP00000019420, Very late activation protein, beta polypeptide, VLA BETA, VLA-4 subunit beta, VLA-BETA, VLAB, VLAbeta
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567)
Western blot analysis of Neuro-2a, using Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Western Blot - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567)
Western blot analysis of A-431, using Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Integrin beta 1 Antibody [ARC52467 + ARC52468] (A307567) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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