Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)

Name: Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572]
Brand: Antibodies.com
SKU: A81034
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0572] antibody to Ionotropic Glutamate receptor 2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572]

Description

Rabbit monoclonal [ARC0572] antibody to Ionotropic Glutamate receptor 2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human GluR2/GRIA2 (P42262).

Sequence

YLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0572

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

99 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AMPA 2, AMPA selective glutamate receptor 2, AMPA-selective glutamate receptor 2, AMPA2, GluA2, GLUR 2, GLUR B, GluR K2, GluR-2, GluR-B, GluR-K2, GLUR2, GLURB, Glutamate receptor 2, Glutamate receptor ionotropic, Glutamate receptor ionotropic AMPA 2, Gria2, GRIA2_HUMAN, HBGR2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)

Western blot analysis of extracts of various cell lines, using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)

Immunofluorescence analysis of SH-SY5Y cells using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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