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Anti-IRS1 (phospho Ser636 + Ser639) Antibody (A11029)

Western Blot - Anti-IRS1 (phospho Ser636 + Ser639) Antibody (A11029) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to IRS1 (phospho Ser636 + Ser639) for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-IRS1 (phospho Ser636 + Ser639) Antibody
Description
Rabbit polyclonal antibody to IRS1 (phospho Ser636 + Ser639).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human
Immunogen
A synthetic phosphorylated peptide around S636 & S639 of human IRS1 (NP_005535.1).
Sequence
MSPKSV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
180 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1_HUMAN, OTTHUMP00000164234
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-IRS1 (phospho Ser636 + Ser639) Antibody (A11029) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-IRS1 (phospho Ser636 + Ser639) Antibody (A11029)
Western blot analysis of extracts of 293T cells, using Anti-IRS1 (phospho Ser636 + Ser639) Antibody (A11029) at 1:500 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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