Anti-ISG15 Antibody (A11423)

Name: Anti-ISG15 Antibody
Brand: Antibodies.com
SKU: A11423
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ISG15 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ISG15 Antibody

Description

Rabbit polyclonal antibody to ISG15.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).

Sequence

MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

G1P2, hUCRP, IFI 15, IFI15, Interferon alpha inducible protein, Interferon induced protein 15, Interferon induced protein IFI-15K, Interferon stimulated protein 15 kDa, Interferon, alpha-inducible protein (clone IFI-15K), Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Interferon-induced 17-kDa/15-kDa protein, IP17, ISG 15, ISG15 ubiquitin like modifier, ISG15_HUMAN, Ubiquitin cross-reactive protein, Ubiquitin-like protein ISG15, UCRP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ISG15 Antibody (A11423)

Western blot analysis of extracts of various cell lines, using Anti-ISG15 Antibody (A11423) at 1:1,000 dilution. Jurkat cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-ISG15 Antibody (A11423)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-ISG15 Antibody (A11423) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ISG15 Antibody (A11423)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-ISG15 Antibody (A11423) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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