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Anti-CD31 Anticorpo [ARC5013-19] (A305350)

Prodotti consigliati per Anti-CD31 Anticorpo

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Anti-CD31 Anticorpo [MEM-05]
Anticorpo monoclonale [MEM-05] di topo contro CD31 per Flow Cytometry, WB, IHC-P e ICC.
Nome
Anti-CD31 Antibody [ARC5013-19]
Descrizione
Rabbit monoclonal [ARC5013-19] antibody to CD31.
Applicazioni
WB, IHC, IP
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000
Reattività
Human
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM1 (NP_000433.4).
Sequenza
AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC5013-19
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
130 - 140 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD31 Antibody [ARC5013-19] (A305350) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD31 Antibody [ARC5013-19] (A305350)
Western blot analysis of extracts of various cell lines, using Anti-CD31 Antibody [ARC5013-19] (A305350) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-CD31 Antibody [ARC5013-19] (A305350) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-CD31 Antibody [ARC5013-19] (A305350)
Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-CD31 Antibody [ARC5013-19] (A305350) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD31 Antibody [ARC5013-19] (A305350) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-CD31 Antibody [ARC5013-19] (A305350)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD31 Antibody [ARC5013-19] (A305350) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot - Anti-CD31 Antibody [ARC5013-19] (A305350) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Western Blot - Anti-CD31 Antibody [ARC5013-19] (A305350)
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-CD31 Antibody [ARC5013-19] (A305350). This Western blot was performed on the immunoprecipitate using Anti-CD31 Antibody [ARC5013-19] (A305350) at a dilution of 1:1000.

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