Anti-CD32 Anticorpo [CCG39] (A280076)

Anti-CD32 Antibody [CCG39]

Mouse monoclonal [CCG39] antibody to CD32.

This antibody recognises the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32 kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.CD32 can function in an inhibtory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009. recognises ovine CD32. For detection of ovine CD32, clone CCG36, which cross reacts with ovine CD32, may be used.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine

This antibody does not cross react with Sheep

Bovine Fc gamma RII-transfected COS7 cells.

Mouse

Monoclonal

CCG39

IgG2a

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine CD32 antibody, clone CCG39, recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32 kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells (Chattha et al. 2010). It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.CD32 can function in an inhibtory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009. Mouse anti Bovine CD32, clone CCG39 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG39 is of interest in that it is specific to bovine CD32 and does not recognizes ovine CD32. For detection of ovine CD32, clone CCG36, which cross reacts with ovine CD32, may be used.

CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIa receptor for (CD32), Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIb receptor for (CD32), Fc fragment of IgG low affinity IIc receptor, Fc fragment of IgG low affinity IIc receptor for (CD32), Fc fragment of IgG, low affinity II, receptor for (CD32), Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIb, receptor (CD32), Fc gamma receptor IIB, Fc gamma receptor IIC, Fc gamma RII a, Fc gamma RII b, Fc gamma RII c, Fc gamma RIIb, Fc gamma RIIc, Fc receptor, IgG, low affinity IIb, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, Fc-GAMMA-RIIC, FCG2, FCG2A_HUMAN, FcGR, FCGR2, FCGR2A, FCGR2A1, FCGR2B, FCGR2C, Fcgr3, Fcgr3a, FcgRII, Fcr-2, Fcr-3, FCRII, FcRII a, FcRII b, FcRII c, FcRII-a, FcRIIC, Fc[g]RII, IGFR2, IgG Fc receptor II a, IgG Fc receptor II b, IgG Fc receptor II beta, IgG Fc receptor II c, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, Immunoglobulin G Fc receptor II, Immunoglobulin G Fc receptor II c, Low affinity immunoglobulin gamma Fc region receptor II, Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17