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Anti-Cytokeratin 7 Anticorpo (A92201)

Nome
Anti-Cytokeratin 7 Antibody
Descrizione
Rabbit polyclonal antibody to Cytokeratin 7.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 240-355 of human Cytokeratin 7 (KRT7) (NP_005547.3).
Sequenza
ISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQE
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
51 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CK 7, CK-7, CK7, Cytokeratin-7, D15Wsu77e, K2C7, K2C7_HUMAN, K7, Keratin 7, Keratin 7, type II, Keratin type II cytoskeletal 7, Keratin, 55K type II cytoskeletal, Keratin, simple epithelial, Keratin, simple epithelial type I, K7, Keratin, type II cytoskeletal 7, Keratin-7, Krt2-7, KRT7, MGC11625, MGC129731, MGC3625, Sarcolectin, SCL, Type II mesothelial keratin K7, Type-II keratin Kb7
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Cytokeratin 7 Antibody (A92201)
Western blot analysis of extracts of HepG2 cells, using Anti-Cytokeratin 7 Antibody (A92201) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Cytokeratin 7 Antibody (A92201)
Western blot analysis of extracts of Rat lung, using Anti-Cytokeratin 7 Antibody (A92201) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Cytokeratin 7 Antibody (A92201) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201)
Immunofluorescence analysis of human lung cancer using Anti-Cytokeratin 7 Antibody (A92201) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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