Recombinant humanized monoclonal antibody to Claudin 6.
Applicazioni
ELISA, Flow Cytometry
Diluizioni consigliate
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reattività
Human
Specie ospite
Humanized
Clonalità
Monoclonal
Isotipo
IgG1
Coniugare
Unconjugated
Purificazione
Affinity chromatography.
Concentrazione
Reconstitution dependent.
Forma del prodotto
Lyophilized
Ricostituzione
Reconstitute with distilled sterile water.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Note generali
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Flow cytometry analysis of 1µg/ml of IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) on Expi293 cells transfected with Human CLDN6 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA plates were pre-coated with 0.5 µg/well purified human CLDN6 MNPs. Serial diluted IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From the above data, the EC50 for IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) binding with Claudin6 MNPs is 34.36 ng/ml.
Flow cytometry analysis of 15µg/ml of IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) on Expi293 cells transfected with Human CLDN9 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Prodotti alternativi a Anti-Claudin 6 Anticorpo (A318880)