This antibody was raised against an intracellular fragment corresponding to amino acids 141-295 of human LIME; a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Applicazioni

IP, Flow Cytometry (Intracellular)

Diluizioni consigliate

Flow Cytometry: 8-12 µg/ml; Intracellular staining.

Reattività

Human

Immunogeno

Bacterially expressed intracellular fragment corresponding to amino acids 141-295 of human LIME protein.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

LIME-06

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein A chromatography.

Concentrazione

1 mg/ml

MW previsto

30 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

dJ583P15.4, FLJ20406, Lck interacting membrane protein, Lck interacting molecule, Lck interacting transmembrane adaptor 1, LIME 1, LIME1, LP8067, RP4 583P15.5

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: Immunoprecipitation - Anti-LIME Antibody [LIME-06] (A86419)

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with Anti-CD3 Antibody [MEM-92] or Anti-CD4 Antibody [MEM-16], and LIME was immunoprecipitated from laurylmaltoside lysates with Anti-LIME Antibody (A86419) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).