Monkey C1q ELISA Kit (A327085)

Monkey C1q ELISA Kit

Monkey C1q ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey C1q in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Monkey C1q ELISA Kit (A327085) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey C1q in serum, plasma, and other biological fluids. An antibody specific for C1q has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the C1q present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-C1q Antibody, which binds the captured C1q present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of C1q captured in each well. The concentration of C1q can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.19 µg/ml

0.31-20 µg/ml

Monkey

No significant cross-reactivity or interference between monkey C1q and analogues was observed.

Intra-assay CV: 4.9%, Inter-assay CV: 5.2%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

C1qa, C1QA_HUMAN, C1QB, C1QC, Complement C1q subcomponent subunit A, Complement C1q subcomponent subunit B, Complement C1q subcomponent subunit C, Complement component 1 q subcomponent alpha polypeptide, Complement component C1q A chain, Complement component C1q B chain, Complement component C1q C chain

Information online is representative. Always refer to the Product Manual inside the kit.