Anti-mTOR (phospho Ser2448) Anticorpo [ARC0094] (A308866)

Name: Anti-mTOR (phospho Ser2448) Antibody [ARC0094]
Brand: Antibodies.com
SKU: A308866
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC0094] di coniglio contro mTOR (phospho Ser2448) per WB.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Tag dei prodotti:

Ribosomal Marker

Descrizione Prodotto

Nome

Anti-mTOR (phospho Ser2448) Antibody [ARC0094]

Descrizione

Rabbit monoclonal [ARC0094] antibody to mTOR (phospho Ser2448).

Applicazioni

Diluizioni consigliate

WB: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S2448 of human mTOR (P42345).

Sequenza

TDSYS

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0094

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

289 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1), FK506 binding protein 12 rapamycin associated protein 1, FK506 binding protein 12 rapamycin associated protein 2, FK506 binding protein 12 rapamycin complex associated protein 1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, MTOR_HUMAN, OTTHUMP00000001983, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin associated protein FRAP2, Rapamycin target protein, Rapamycin target protein 1, RAPT1, Serine/threonine-protein kinase mTOR

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Western blot analysis of extracts of 293T cells, using Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866) at 1:1,000 dilution. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Western blot analysis of extracts of 293T cells, using Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Western Blot - Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866) at 1:1,000 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.