Anti-Nrf2 Anticorpo [ARC0806] (A309426)

Name: Anti-Nrf2 Antibody [ARC0806]
Brand: Antibodies.com
SKU: A309426

Clicca sull'immagine per la visualizzazione estesa

Questo prodotto è fuori produzione

Anti-Nrf2 Anticorpo [ARC0806] (A309426) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-Nrf2 Anticorpi.

Descrizione Prodotto

Nome

Anti-Nrf2 Antibody [ARC0806]

Descrizione

Rabbit monoclonal [ARC0806] antibody to Nrf2.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reattività

Human

Immunogeno

A synthesized peptide derived from human NRF2.

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0806

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

97 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

erythroid derived 2, HEBP1, like 2, NF E2 related factor 2, NF-E2-related factor 2, NF2L2_HUMAN, NFE2 related factor 2, NFE2-related factor 2, Nfe2l2, Nrf 2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, nuclear factor erythroid 2 like 2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-Nrf2 Antibody [ARC0806] (A309426)

Western blot analysis of extracts of HeLa cells, using Anti-Nrf2 Antibody [ARC0806] (A309426) at 1:500 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-Nrf2 Antibody [ARC0806] (A309426)

Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using Anti-Nrf2 Antibody [ARC0806] (A309426) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Immunofluorescence - Anti-Nrf2 Antibody [ARC0806] (A309426)

Immunofluorescence analysis of HepG2 using Anti-Nrf2 Antibody [ARC0806] (A309426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).