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Anti-Nucleolin Anticorpo (A15046)

Western Blot - Anti-Nucleolin Antibody (A15046) - Antibodies.com Immunohistochemistry - Anti-Nucleolin Antibody (A15046) - Antibodies.com
$335
Product Datasheet

Anticorpo policlonale di coniglio contro Nucleolin per WB e IHC.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Nucleolin Antibody
Descrizione
Rabbit polyclonal antibody to Nucleolin.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reattività
Human, Mouse
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 300-466 of human NCL (NP_005372.2).
Sequenza
GTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGE
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
110 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Nucleolin Antibody (A15046) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Nucleolin Antibody (A15046)
Western blot analysis of extracts of various cell lines, using Anti-Nucleolin Antibody (A15046) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Nucleolin Antibody (A15046) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-Nucleolin Antibody (A15046)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Nucleolin Antibody (A15046) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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