Ricombinante Anti-Nucleolin Anticorpo [NCL/7014R] (A278625)

Nome
Anti-Nucleolin Antibody [NCL/7014R] - BSA and Azide free
Descrizione
Recombinant rabbit monoclonal [NCL/7014R] antibody to Nucleolin.
Specificità
This antibody recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Applicazioni
Flow Cytometry, IF, WB, IHC-P
Diluizioni consigliate
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reattività
Human
Reattività incrociata
This antibody does not cross react with Mouse, Feline, Bovine, Canine, or Horse.
Immunogeno
Recombinant full-length human NCL protein.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
NCL/7014R
Isotipo
IgG
Catene leggere
kappa
Coniugare

Unconjugated

Purificazione
Protein A/G chromatography.
Concentrazione
1 mg/ml
Peso molecolare
76 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Note generali
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Nucleolin Antibody [NCL/7014R] (A278037).
Sinonimi
C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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