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Anti-S100A4 Anticorpo [ARC0475] (A305282)

Nome
Anti-S100A4 Antibody [ARC0475]
Descrizione
Rabbit monoclonal [ARC0475] antibody to S100A4.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 2-101 of human S100A4 (P26447).
Sequenza
ACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC0475
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
12 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
18A2, 42A, calcium Placental protein, Calvasculin, CAPL, Fibroblast specific protein, Fibroblast specific protein 1, Fibroblast specific protein 1 (FSP1), FSP1, Leukemia multidrug resistance associated protein, Malignant transformation suppression 1, Malignant transformation suppression 1 (MTS1), Metastasin, MTS1, OTTHUMP00000015467, OTTHUMP00000015468, P9KA, PEL98, Placental calcium-binding protein, Protein Mts1, Protein S100 A4, Protein S100-A4, S100 calcium binding protein A4, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S10A4_HUMAN
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282)
Western blot analysis of extracts of various cell lines, using Anti-S100A4 Antibody [ARC0475] (A305282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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