Anti-SMAD5 (phospho Ser463 + Ser465) Anticorpo (A308813)

Name: Anti-SMAD5 (phospho Ser463 + Ser465) Antibody
Brand: Antibodies.com
SKU: A308813
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro SMAD5 (phospho Ser463 + Ser465) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-SMAD5 (phospho Ser463 + Ser465) Antibody

Descrizione

Rabbit polyclonal antibody to SMAD5 (phospho Ser463 + Ser465).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S463 & S465 of human Phospho-Smad5-S463/S465 (NP_005894.3).

Sequenza

SSVS

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Western blot analysis of extracts of NIH/3T3, using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at 1:600 dilution. NIH/3T3 cells were treated by BMP4 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.