Anti-SMAD5 (phospho Ser465) Anticorpo [ARC59204] (A309476)

Name: Anti-SMAD5 (phospho Ser465) Antibody [ARC59204]
Brand: Antibodies.com
SKU: A309476
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC59204] di coniglio contro SMAD5 (phospho Ser465) per WB.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-SMAD5 (phospho Ser465) Antibody [ARC59204]

Descrizione

Rabbit monoclonal [ARC59204] antibody to SMAD5 (phospho Ser465).

Applicazioni

Diluizioni consigliate

WB: 1:1,000-1:5,000

Reattività

Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S465 of human Smad5.

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC59204

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

60 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Western Blot - Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Western blot analysis of NIH/3T3, using Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476) at 1:2,000 dilution. NIH/3T3 cells were treated by BMP4(50ng/ml) at 37°C for 6 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Western blot analysis of C6, using Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476) at 1:2,000 dilution. C6 cells were treated by MG132(50 µM) and TGFbetaat 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.