Rabbit polyclonal antibody to ITGB4 (Phospho-Tyr1510)
Specificity
The antibody detects endogenous levels of ITGB4 only when phosphorylated at tyrosine 1510.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine1510(R-D-Y(p)-S-T) derived from Human ITGB4 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using ITGB4 (Phospho-Tyr1510) Antibody #11698.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ITGB4 (Phospho-Tyr1510) antibody #11698 (left)or the same antibody preincubated with blocking peptide (right).
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