Rabbit polyclonal antibody to JAK1 (phospho Tyr1022 + Tyr1023).
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around Y1022 & Y1023 of human JAK1 (NP_002218.2).
Sequence
KEYYTV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
130 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
JAK 1, JAK 1A, JAK 1B, JAK-1, JAK1A, JAK1B, JAK1_HUMAN, Janus kinase 1, Janus kinase 1 (a protein tyrosine kinase), JTK3, Tyrosine protein kinase JAK 1, Tyrosine protein kinase JAK1, Tyrosine-protein kinase JAK1
Figure 1: Western Blot - Anti-JAK1 (phospho Tyr1022 + Tyr1023) Antibody (A16681)
Western blot analysis of extracts of RAW264. 7 cells, using Anti-JAK1 (phospho Tyr1022 + Tyr1023) Antibody (A16681) at 1:1,000 dilution. RAW264. 7 cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-JAK1 (phospho Tyr1022 + Tyr1023) Antibody (A16681) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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