Anti-JNK1 (phospho Thr183/Tyr185)+ JNK2 (phospho Thr183/Tyr185) + JNK3 (phospho Thr221/Tyr223) Antibody (A283687)

Anti-JNK1 (phospho Thr183/Tyr185)+ JNK2 (phospho Thr183/Tyr185) + JNK3 (phospho Thr221/Tyr223) Antibody

Rabbit polyclonal antibody to JNK1 (phospho Thr183/Tyr185)+ JNK2 (phospho Thr183/Tyr185) + JNK3 (phospho Thr221/Tyr223).

This antibody recognises mitogen activated protein kinase (MAPK) 8 and 9, also known as c-Jun N-terminal kinases 1 and 2 or stress activated protein kinases 1 and 2, when phosphorylated on threonine 183 and tyrosine 185. This antibody also recognises MAPK10, also known as c-Jun N-terminal kinase 3 or stress activated protein kinase 3, when phosphorylated on threonine 221 and tyrosine 223.As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012, Bogoyevitch and Kobe 2006).Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family. The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis.

WB: 1:500 - 1:2,000

Rat, Human, Mouse

Phospho-specific peptide corresponding to residues surrounding Thr183/Tyr185 of human MAPK8/MAPK9 and to residues surrounding Thr221/Tyr223 of human MAPK10.

Rabbit

Polyclonal

IgG

Lot Specific

Liquid

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.02% Sodium Azide.

Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody recognizes mitogen activated protein kinase (MAPK) 8 and 9, also known as c-Jun N-terminal kinases 1 and 2 or stress activated protein kinases 1 and 2, when phosphorylated on threonine 183 and tyrosine 185. This antibody also recognizes MAPK10, also known as c-Jun N-terminal kinase 3 or stress activated protein kinase 3, when phosphorylated on threonine 221 and tyrosine 223.As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012, Bogoyevitch and Kobe 2006).Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family (Guma and Firestein 2012). The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis (Guma and Firestein 2012).