Anti-KCNK3 Antibody [S374-48] (A304820)

Name: Anti-KCNK3 Antibody [S374-48]
Brand: Antibodies.com
SKU: A304820
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S374-48] antibody to KCNK3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-KCNK3 Antibody [S374-48]

Description

Mouse monoclonal [S374-48] antibody to KCNK3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with TASK3.

Immunogen

Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3..

Host

Mouse

Clonality

Monoclonal

Clone ID

S374-48

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Acid sensitive potassium channel protein TASK, Acid sensitive potassium channel protein TASK 1, Acid-sensitive potassium channel protein TASK-1, Cardiac two pore background K(+) channel, cTBAK 1, K2p3.1, KCNK3_HUMAN, OAT1, Potassium channel subfamily K member 3, potassium channel, subfamily K, member 3, Potassium inwardly rectifying channel subfamily K member 3, PPH4, rTASK, TASK, TASK 1, TBAK1, TWIK related acid sensitive K+ channel, TWIK-related acid-sensitive K(+) channel 1, Two P domain potassium channel, Two pore K(+) channel KT3., Two pore K(+) channel KT3.1, Two pore potassium channel KT3.1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-KCNK3 Antibody [S374-48] (A304820)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-KCNK3 Antibody [S374-48] (A304820), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel Antibody (D) Composite.

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Figure 2: Western Blot - Anti-KCNK3 Antibody [S374-48] (A304820)

Western blot analysis of rat brain membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Anti-KCNK3 Antibody [S374-48] (A304820) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~50 kDa.

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Figure 3: Immunofluorescence - Anti-KCNK3 Antibody [S374-48] (A304820)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-KCNK3 Antibody [S374-48] (A304820), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TASK1 Potassium Channel Antibody. (D) Composite.

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