Anti-KCNQ4 Antibody [N43/6] (A305021)

Name: Anti-KCNQ4 Antibody [N43/6]
Brand: Antibodies.com
SKU: A305021
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [N43/6] antibody to KCNQ4 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-KCNQ4 Antibody [N43/6]

Description

Mouse monoclonal [N43/6] antibody to KCNQ4.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 2-77 of human KCNQ4 .

Host

Mouse

Clonality

Monoclonal

Clone ID

N43/6

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~77 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S43-6.

Synonyms

DFNA 2, DFNA2, KCNQ 4, KCNQ4_HUMAN, KQT like 4, KQT-like 4, KV7.4, Potassium channel KQT like 4, Potassium channel subunit alpha KvLQT4, Potassium voltage gated channel KQT like protein 4, Potassium voltage gated channel KQT like subfamily member 4, Potassium voltage gated channel subfamily KQT member 4, Potassium voltage-gated channel subfamily KQT member 4, Voltage gated potassium channel subunit Kv7.4, Voltage-gated potassium channel subunit Kv7.4

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-KCNQ4 Antibody [N43/6] (A305021)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-KCNQ4 Antibody [N43/6] (A305021), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ4 Antibody (D) Composite.

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Figure 2: Immunohistochemistry - Anti-KCNQ4 Antibody [N43/6] (A305021)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-KCNQ4 Antibody [N43/6] (A305021) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

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Figure 3: Western Blot - Anti-KCNQ4 Antibody [N43/6] (A305021)

Western blot analysis of rat tissue lysate showing detection of KCNQ4 protein using Anti-KCNQ4 Antibody [N43/6] (A305021) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 4: Immunohistochemistry - Anti-KCNQ4 Antibody [N43/6] (A305021)

Immunohistochemistry analysis of mouse frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-KCNQ4 Antibody [N43/6] (A305021) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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