Anti-Keap1 Antibody (A309232)

Name: Anti-Keap1 Antibody
Brand: Antibodies.com
SKU: A309232
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Keap1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Keap1 Antibody

Description

Rabbit polyclonal antibody to Keap1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KEAP1 (NP_036421.2).

Sequence

YLVKIFEELTLHKPTQVMPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSDGTWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 - 64 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cytosolic inhibitor of Nrf2, INrf 2, INrf2, Keap 1, KEAP1_HUMAN, Kelch like ECH associated protein 1, Kelch like family member 19, Kelch like protein 19, Kelch-like ECH-associated protein 1, Kelch-like protein 19, KIAA0132, KLHL 19, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Keap1 Antibody (A309232)

Western blot analysis of extracts from wild type(WT) and KEAP1 Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-Keap1 Antibody (A309232) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-Keap1 Antibody (A309232)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Keap1 Antibody (A309232) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Keap1 Antibody (A309232)

Immunofluorescence analysis of PC-12 cells using Anti-Keap1 Antibody (A309232) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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