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Anti-Keratin 36 Antibody (A90358)

Western Blot - Anti-Keratin 36 Antibody (A90358) - Antibodies.com Immunohistochemistry - Anti-Keratin 36 Antibody (A90358) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Keratin 36 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Keratin 36 Antibody
Description
Rabbit polyclonal antibody to Keratin 36.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human KRT36 (NP_003762.1).
Sequence
LNDRLANYLEKVRQLERENAELESRIQEWYEFQIPYICPDYQSYFKTIEDFQQKILLTKSENARLVLQIDNAKLAADDFRTKYETELSLRQLVEADINGLR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
52 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HA6, Hair keratin, Hair keratin, type I Ha6, HHA6, HKA6, K36, Keratin, Keratin hair acidic 6, Keratin type I cuticular Ha6, keratin, hair, acidic, 6, Keratin, hard, type I, Keratin, hard, type I, 6, keratin, type I cuticular Ha6, Keratin-36, Krt36, KRT36_HUMAN, KRTHA6, type I cuticular Ha6, type I Ha6
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Keratin 36 Antibody (A90358) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Keratin 36 Antibody (A90358)
Western blot analysis of extracts of various cell lines, using Anti-Keratin 36 Antibody (A90358) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-Keratin 36 Antibody (A90358) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Keratin 36 Antibody (A90358)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Keratin 36 Antibody (A90358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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