Specificity
The antibody recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody recognizes kinesin bound to taxol-stabilized microtubules.