Anti-Kir2.1 Antibody [S112] (A304786)

Name: Anti-Kir2.1 Antibody [S112]
Brand: Antibodies.com
SKU: A304786
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S112] antibody to Kir2.1 for WB, IHC, ICC/IF and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Kir2.1 Antibody [S112]

Description

Mouse monoclonal [S112] antibody to Kir2.1.

Applications

WB, IHC, ICC/IF, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

This antibody does not cross-react with Kir2.2 or Kir2.3.

Immunogen

Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S112

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cardiac inward rectifier potassium channel, HHBIRK 1, HHBIRK1, HHIRK 1, HHIRK1, HIRK 1, hIRK1, Inward rectifier K, Inward rectifier K(+) channel Kir2.1, Inward rectifier potassium channel 2, inwardly rectifying subfamily J member 2, IRK 1, IRK-1, IRK1, IRK2_HUMAN, KCNJ2, LQT 7, LQT7, Potassium channel, Potassium channel inwardly rectifying subfamily J member 2, Potassium inwardly rectifying channel J2, Potassium inwardly rectifying channel subfamily J member 2, SQT 3, SQT3

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-Kir2.1 Antibody [S112] (A304786)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Kir2.1 Antibody [S112] (A304786), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.

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Figure 2: Immunohistochemistry - Anti-Kir2.1 Antibody [S112] (A304786)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-Kir2.1 Antibody [S112] (A304786) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

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Figure 3: Western Blot - Anti-Kir2.1 Antibody [S112] (A304786)

Western blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Anti-Kir2.1 Antibody [S112] (A304786) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 4: Immunohistochemistry - Anti-Kir2.1 Antibody [S112] (A304786)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-Kir2.1 Antibody [S112] (A304786) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Nuclear expression in the epidermis and hair follicles.

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