KNTC2 Cell Based ELISA Kit (A103458)

KNTC2 Cell Based ELISA Kit

Cell-based (qualitative)

This KNTC2 Cell Based ELISA Kit allows for the detection of KNTC2 and the effects that certain stimulation conditions have on KNTC2 expression in different cell lines. Qualitative determination of KNTC2 concentration is achieved by an indirect ELISA format. In essence, the KNTC2 is captured by Anti-KNTC2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this KNTC2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

from Entrez Gene ;, HEC, HEC1, Highly expressed in cancer, Highly expressed in cancer protein, Highly expressed in cancer rich in leucine heptad repeats, HsHec1, hsNDC80, Kinetochore associated 2, Kinetochore associated protein 2, Kinetochore protein Hec1, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, ndc80, NDC80 homolog kinetochore complex component, NDC80 kinetochore complex component homolog, NDC80, S. cerevisiae, homolog of, NDC80_HUMAN, Retinoblastoma associated protein HEC, Retinoblastoma-associated protein HEC, TID3