Anti-KRAS Antibody [ARC59916] (A309944)

Name: Anti-KRAS Antibody [ARC59916]
Brand: Antibodies.com
SKU: A309944

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This product is discontinued

Anti-KRAS Antibody [ARC59916] (A309944) has been discontinued and is no longer available.

View all Anti-KRAS Antibodies.

Specifications

Name

Anti-KRAS Antibody [ARC59916]

Description

Rabbit monoclonal [ARC59916] antibody to KRAS.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human KRAS.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59916

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

c Ki ras2, c Kirsten ras protein, c-K-ras, c-Ki-ras, Cellular c Ki ras2 proto oncogene, Cellular transforming proto oncogene, CFC2, cK Ras, GTPase KRas, K RAS p21 protein, K RAS2A, K RAS2B, K RAS4A, K RAS4B, K-Ras 2, KI RAS, Ki-Ras, KIRSTEN MURINE SARCOMA VIRUS 2, Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog, Kirsten rat sarcoma viral oncogene homolog, KRAS proto oncogene, GTPase, KRAS1, KRAS2, N-terminally processed, NS, NS3, Oncogene KRAS2, p21ras, PR310 c K ras oncogene, PR310 cK ras oncogene, RALD, RASK2, RASK_HUMAN, Transforming protein p21, v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v Ki ras2 Kirsten rat sarcoma viral oncogene homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-KRAS Antibody [ARC59916] (A309944)

Western blot analysis of various lysates, using Anti-KRAS Antibody [ARC59916] (A309944) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 45s.

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Figure 2: Western Blot - Anti-KRAS Antibody [ARC59916] (A309944)

Western blot analysis of extracts from wild type(WT) and KRAS Rabbit mAb knockout (KO) 293T(KO) cells, using Anti-KRAS Antibody [ARC59916] (A309944) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 45s.

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Figure 3: Immunohistochemistry - Anti-KRAS Antibody [ARC59916] (A309944)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-KRAS Antibody [ARC59916] (A309944) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-KRAS Antibody [ARC59916] (A309944)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-KRAS Antibody [ARC59916] (A309944) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.