Anti-Ku80 Antibody [ARC0706] (A307476)

Name: Anti-Ku80 Antibody [ARC0706]
Brand: Antibodies.com
SKU: A307476
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0706] antibody to Ku80 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Ku80 Antibody [ARC0706]

Description

Rabbit monoclonal [ARC0706] antibody to Ku80.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80 (P13010).

Sequence

MKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0706

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

86 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II, ATP dependent DNA helicase II 80 kDa subunit, ATP dependent DNA helicase II 86 Kd subunit, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box binding factor 85 kDa, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP 1, KARP1, Ku 80, Ku autoantigen 80kDa, Ku86, Ku86 autoantigen related protein 1, KUB 2, KUB2, Lupus Ku autoantigen protein p86, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining), X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5, Xray repair complementing defective repair in Chinese hamster cells 5, XRCC 5, XRCC5, XRCC5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Ku80 Antibody [ARC0706] (A307476)

Western blot analysis of extracts of various cell lines, using Anti-Ku80 Antibody [ARC0706] (A307476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Ku80 Antibody [ARC0706] (A307476)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Ku80 Antibody [ARC0706] (A307476) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Ku80 Antibody [ARC0706] (A307476)

Confocal imaging of HeLa cells using Anti-Ku80 Antibody [ARC0706] (A307476), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 4: Western Blot - Anti-Ku80 Antibody [ARC0706] (A307476)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Ku80 Antibody [ARC0706] (A307476). This Western blot was performed on the immunoprecipitate using Anti-Ku80 Antibody [ARC0706] (A307476) at a dilution of 1:1000.

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