Recombinant human monoclonal [Relatlimab Biosimilar] antibody to LAG-3.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG4
Conjugate
Unconjugated
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze / thaw cycles.
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human LAG-3 Protein (6×His Tag) (A318223) which can bind Anti-LAG-3 Antibody [Relatlimab Biosimilar] - Azide free (A318896) in a linear range of 0.6-100 µg/ml.
Flow cytometry analysis with Anti-LAG-3 Antibody [Relatlimab Biosimilar] - Azide free (A318896) at 1 µg/ml on Expi293 cells transfected with human LAG3 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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