Anti-LAMP1 Antibody [ARC52154] (A305368)

Name: Anti-LAMP1 Antibody [ARC52154]
Brand: Antibodies.com
SKU: A305368

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This product is discontinued

Anti-LAMP1 Antibody [ARC52154] (A305368) has been discontinued and is no longer available.

View all Anti-LAMP1 Antibodies.

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Specifications

Name

Anti-LAMP1 Antibody [ARC52154]

Description

Rabbit monoclonal [ARC52154] antibody to LAMP1.

Applications

WB, IHC, ICC/IF, Flow Cytometry

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, Flow Cytometry: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-382 of human LAMP1 (NP_005552.3).

Sequence

AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52154

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa / 90 - 120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD107 antigen like family member A, CD107 antigen-like family member A, CD107a, CD107a antigen, LAMP 1, LAMP-1, LAMP1_HUMAN, LAMPA, LGP120, lgpA, Lysosomal Associated Membrane Protein 1, Lysosomal membrane glycoprotein 120KD, Lysosome associated membrane glycoprotein 1, Lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1, OTTHUMP00000040663

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-LAMP1 Antibody [ARC52154] (A305368)

Western blot analysis of extracts of U-251MG cells, using Anti-LAMP1 Antibody [ARC52154] (A305368) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 2: Western Blot - Anti-LAMP1 Antibody [ARC52154] (A305368)

Western blot analysis of extracts from wild type (WT) and LAMP1 knockout (KO) HeLa and HeLa-LAMP1-2E4 cells, using Anti-LAMP1 Antibody [ARC52154] (A305368) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 3: Immunohistochemistry - Anti-LAMP1 Antibody [ARC52154] (A305368)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-LAMP1 Antibody [ARC52154] (A305368) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-LAMP1 Antibody [ARC52154] (A305368)

Immunofluorescence analysis of HeLa and HeLa-LAMP1-2E4(çº¯åˆå) cells using Anti-LAMP1 Antibody [ARC52154] (A305368) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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