Anti-LDL Receptor Antibody [ARC51372] (A308994)

Name: Anti-LDL Receptor Antibody [ARC51372]
Brand: Antibodies.com
SKU: A308994

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This product is discontinued

Anti-LDL Receptor Antibody [ARC51372] (A308994) has been discontinued and is no longer available.

View all Anti-LDL Receptor Antibodies.

Specifications

Name

Anti-LDL Receptor Antibody [ARC51372]

Description

Rabbit monoclonal [ARC51372] antibody to LDL Receptor.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).

Sequence

TVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51372

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 - 160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FH, FHC, LDL R, LDLCQ2, Ldlr, LDLR_HUMAN, Low Density Lipoprotein Receptor, Low density lipoprotein receptor class A domain containing protein 3, Low density lipoprotein receptor familial hypercholesterolemia, Low-density lipoprotein receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-LDL Receptor Antibody [ARC51372] (A308994)

Western blot analysis of extracts of various cell lines, using Anti-LDL Receptor Antibody [ARC51372] (A308994) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-LDL Receptor Antibody [ARC51372] (A308994)

Western blot analysis of extracts of A-431 cells, using Anti-LDL Receptor Antibody [ARC51372] (A308994) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-LDL Receptor Antibody [ARC51372] (A308994)

Immunofluorescence analysis of HepG2 using Anti-LDL Receptor Antibody [ARC51372] (A308994) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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