This antibody was raised against an intracellular fragment corresponding to amino acids 141-295 of human LIME; a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Applications

IP, Flow Cytometry (Intracellular)

Dilutions

Flow Cytometry: 8-12 µg/ml; Intracellular staining.

Reactivity

Human

Immunogen

Bacterially expressed intracellular fragment corresponding to amino acids 141-295 of human LIME protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

LIME-06

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Predicted MW

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

dJ583P15.4, FLJ20406, Lck interacting membrane protein, Lck interacting molecule, Lck interacting transmembrane adaptor 1, LIME 1, LIME1, LP8067, RP4 583P15.5

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

Enlarge Image

Figure 1: Immunoprecipitation - Anti-LIME Antibody [LIME-06] (A86419)

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with Anti-CD3 Antibody [MEM-92] or Anti-CD4 Antibody [MEM-16], and LIME was immunoprecipitated from laurylmaltoside lysates with Anti-LIME Antibody (A86419) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).

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