Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398)

Name: Anti-Lin28A Antibody [ARC53062 + ARC53068]
Brand: Antibodies.com
SKU: A309398

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This product is discontinued

Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398) has been discontinued and is no longer available.

View all Anti-Lin28A Antibodies.

Specifications

Name

Anti-Lin28A Antibody [ARC53062 + ARC53068]

Description

Rabbit Oligoclonal [ARC53062 + ARC53068] antibody to Lin28A.

Applications

WB, IHC

Dilutions

WB: 1:10,000-1:70,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human LIN28A.

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC53062 + ARC53068

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CSDD1, FLJ12457, LIN 28, Lin 28 homolog A (C. elegans), Lin-28A, LIN28, LN28A_HUMAN, Protein lin-28 homolog A, ZCCHC1, zinc finger CCHC domain containing 1, Zinc finger CCHC domain-containing protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398)

Western blot analysis of various lysates, using Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398)

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Figure 3: Immunohistochemistry - Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398)

Immunohistochemistry analysis of paraffin-embedded human seminoma using Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Lin28A Antibody [ARC53062 + ARC53068] (A309398) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.